PeakFit

What is PeakFit?

PeakFit uses three procedures to automatically place hidden peaks; although each is a strong solution, a method can work better with some datasets than with others.

The Residuals procedure initially places peaks by finding maximum locations in a smoothed data flow. Hidden peaks are then optionally added where spikes occur in the residuals.

The Second Derivative procedure looks for minimal locations within a data stream of the second smoothed derivative. These local minimums often reveal hidden peaks.

The deconvolution procedure uses a Gaussian response function with a Fourier deconvolution/filtering algorithm. A spectrum successfully defiantwill consist of "sharp" peaks of equivalent area. The goal is to increase the hidden peaks so that each represents a maximum location.

Overview

Full graphical placement of peaks.

If PeakFit's auto-positioning features fail on extremely complicated or noisy data, you can place and adjust the peaks graphically with just a few mouse clicks. Each function placed has "anchors" that adjust even the most complex functions, automatically changing the numeric parameters specific to that function. PeakFit's graphical positioning options treat even the most complex peaks as smoothly as gaussians.

PeakFit offers sophisticated data manipulation.

With PeakFit's visual FFT filter, you can inspect your data flow in the Fourier domain and zero higher frequency points - and see your results immediately in the time domain. This smoothing technique enables excellent noise reduction while maintaining the integrity of the original data flow.

PeakFit also includes an automated FFT method, as well as Gaussian convolution, the Savitzky-Golay method, and the Loess algorithm for smoothing. AI experts in all smoothing options and other parts of the program automatically help you set many adjustments. And PeakFit even has a digital data enhancer that helps analyze your sparse data. Only PeakFit offers so many different methods of data manipulation.

Highly advanced baseline subtraction

PeakFit's nonparametric baseline adjustment routine easily removes the complex background of a DNA electrophoresis sample. PeakFit can also subtract eight other inline baseline equations, or you can subtract any baseline you have developed and stored in a file.

Data output and publication quality charts

Each publication-quality chart (see above) was created using PeakFit's built-in graphics engine - which now includes print preview and file and clipboard export options. The numeric output is customizable so that you see only the content you want.

PeakFit saves your precious search time

For most datasets, PeakFit does all the work for you. What used to take hours now takes minutes – with just a few mouse clicks! It's so easy that new yorkers can learn how to use PeakFit quickly. And if you have extremely complex or noisy datasets, the sophistication and depth of PeakFit's data manipulation techniques are second to none.

Hardware

Processor 486 or higher

16 MB of RAM required (32 MB or more recommended)

10 MB of hard disk space

Programmes

Windows 10, 8.x, 7, Windows Vista, 95, 98, NT e XP (32 bits)